Does anyone have experience in semen analysis of wistar rats?

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M. A. El-Sayed

Hi Dr yugesh,

I analyse semen by;

microscope (motility,normal and abnormal,.....) and by photometer was estmaited (numbers,ejaculation voulum,Ph,....... and soo on

Stefan S Du Plessis

We use epididymal retrieved sperm from Wistar rats.

Unfortunately the sperm very quickly loose there motility, but we analyse concentration and motility via CASA on a Sperm Class Analyser (SCA Microptic) system. For morphological analysis we stain with SpermBlue and also use the Morphology module of the SCA system.

Also refer to a Manuscript soon to appear in Biotechnic & Histochemistry - Automated analysis of rat sperm morphometry and morphology: Moving towards cut-off values for normal sperm morphology and toxicology.