Does anyone have experience do mouse lung ischemia/reperfusion?

More Jeffrey J. Baust's questions See All

Resuspending large bacterial pellets using blender?

I do an 8L induction at 18C for 16-20 hours in TB media. I routinely harvest ~50-80g of bacteria. I freeze my pellets at -20 for > 3 hours after harvesting, and have been using a 1kW blender to...

04 September 2023 1,047 3 View

What are your recommendations in optimizing p53 IHC using Leica Biosystems for the detection of TP53 mutation in endometrial carcinomas?

I will be conducting a study on p53 IHC optimization for Endometrial Ca with subsequent TP53 mutational analysis here in our institution. We will be using RTU antibody (DO-7) but with Leica...

02 February 2023 9,940 0 View

What Universities in the USA have the capability for primary culture of pituitary and thyroid from rats and humans?

I'm looking for a USA-based University that is able to culture rat and human pituitary and thyroid glands to evaluate effects of chemicals on TSH and T3/T4 production. It would be beneficial if...

24 January 2023 8,488 0 View

Recommendation for resources in anthropology (and otherwise) on the changing nature of public space?

As tourism grows and tourists come to dominate public spaces, the value/use of those spaces to native-born populations is shifting. This is a rather new topic for me, but looking for resources...

24 October 2022 1,599 2 View

What could be a role of System-of-Systems (SoS) in multicellular biology?

How are trillions of cells in a maturing biologic entity coordinated so that the entity can act as a singular "whole" in order to survive?

21 June 2022 3,821 0 View

Can Phosphorus content in plants not reduce under drought stress?

My research investigated the impact of water stress on plant nutrition. Phosphorus content was stable regardless of increasing PEG concentration. Is there any valid explanation on why as i am...

07 June 2022 4,811 7 View

Is there a method of competing risk analysis that does not assume proportional hazards?

The cause-specific hazard model and the Fine-Gray subdistribution model are well known methods of competing risk analysis, but both assumes proportional hazards. Is there any method of handling...

02 June 2022 4,434 1 View

Where could I find a list of S class L-functions that have non-critical zeros in the critical strip?

I am looking for S class L-functions, namely Maass forms, that do not satisfy the RH. Could anyone provide one or two, preferably functional equations? I had read some papers that were written on...

18 January 2022 6,880 0 View

Conviron growth chamber will not continue to run programmed schedule?

I am student at Humboldt State University, I have been trying to get one of our growth chambers, a conviron E7/2 or E8, back into operation. The problem I am running into is that once I set up a...

26 August 2021 5,200 0 View

Historical lists or indices of magnetic-storm sudden commencements and coronal holes?

Hello, I'm interested in looking at long-term (100+ year) measures of magnetic storm sudden commencements and, also, coronal holes. Is there a convenient list of SSCs? Have coronal holes been...

06 August 2021 4,699 1 View

Fixation Problem with cancer cells?

Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...

19 July 2024 3,906 0 View

Does egg white interfere with any histology staining?

I have mouse lung tissue which is mounted with egg whites.I need to stain my tissues with PAS and HE. Can egg whites interfere with these stainings?

02 July 2024 8,261 0 View

Can an Ischemic Buffer Alone Effectively Mimic Hypoxia in an In Vitro Cardiac Cell Hypoxia-Reoxygenation Model?

I am working on an in-vitro model of hypoxia-reoxygenation (H/R) in cardiac cells (H9c2). Due to the unavailability of a hypoxic incubator, I am exploring alternative methods to create hypoxic...

30 June 2024 1,234 2 View

How does prolonged ischemia lead to myocardial necrosis?

Comprehensive Overview of Myocardial Infarction: Etiology, E... How does prolonged ischemia lead to myocardial necrosis?

15 June 2024 6,589 1 View

What kind of cell culture contamination is it?

Hi everyone, I need help and yuoue experience!!! What kind of cell culture contamination is it? Video attached: The cells are lung cancer cells that are thawed from -80°C fridge and we known that...

04 June 2024 9,619 2 View

How to download Lung cancer (LUSC) gene expression data from TCGA(https://portal.gdc.cancer.gov/repository)?

How to download Lung cancer (LUSC) gene expression data which includes tumor tissue samples and corresponding control tissue samples from TCGA(https://portal.gdc.cancer.gov/repository). Any...

13 May 2024 2,481 1 View

How to optimize RIN values from lung tissue for RNA-seq?

I've been trying to extract RNA from mouse lung tissues (normal and tumour) and slowly improving the yield and purity measured via Nanodrop and Qubit (I'm consistently getting 260/280 ratios of ~2...

21 March 2024 113 5 View

How can I overcome the overfitting problem?

I have a dataset from lung cancer images with 163 samples (2D images). I use the fine-tuning of deep learning algorithms to classify samples, but the validation loss did not decrease. I augmented...

21 March 2024 8,344 6 View

How can I determine the IC50 drug concentration to normal lung cells?

Dear All, I need to standardize synthesized drug concentration (IC50) for my in vitro experiment but I can't adopt MTT, LDH, or trypan blue kind of assay because I'm working on normal...

20 February 2024 947 3 View

How to separate dead cells from live cells when propagating suspension cells?

I am growing small cell lung cancer suspension cells. How do I separate dead cells from live cells while splitting the cells after confluency in flask?

28 January 2024 8,818 6 View

Jeffrey J. Baust

Thank you for your input Sir.

Cheers!

jjb

Matt Giangola

Results can also vary depending on the temperature of the mouse. A heating pad helps in hemorrhagic shock and some I/R models. Also, depending on the method of clamping (tie, clamp, endoloop) ensuring not to cause a major thrombus/embolism is key.

Thank you Matt,

I git the model up and running with 90% success. I was making it harder than was.