Does anyone have experience building search engine with Zend/Lucene? Did you find some pitfalls?

More Beatriz Fernández Reuter's questions See All

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

¿Where to buy the Aspergillus oryzae NSAR1 strain?

Greetings: I am looking to buy/obtain the auxotrophic strain Aspergillus oryzae NSAR1, but I just have found one culture collection from Japan but haven't received any answer. Could you provide...

11 July 2024 6,847 0 View

How can I improve Gibson Assembly's efficiency?

In my undergraduate research project, I require an integrative plasmid containing GFP with an antibiotic resistance that can't be kanamycin. In our lab, all of our integrative-GFP-containing...

14 May 2024 9,861 3 View

How to improve 260/230 ratio in rna extraction for PCR?

The 260/280 ratio is ok between 1.8 and 2.0 however we were unable to advance due to the 260/230 ratio

13 May 2024 7,643 3 View

What is the optim temperature for protein denaturation in supernatants??

Hello! I´m developing a sandwich ELISA. My assay recognizes protein denaturalized very well, but there isn´t recognition when it is in its native form. So maybe if I heat the sample I could detect...

18 April 2024 5,671 3 View

MorphoJ is not giving me the results of Canonical Variate Analysis. What I do?

When I request MorphoJ to do a CVA, it gives to me only the graphics, but no results.

27 March 2024 1,672 1 View

Problems isolating human neutrophils using Percoll - Erythrocytes not pelleting?

Hello all, I present here a problem to see if someone has encountered it and can provide some clarity. We are trying to isolate neutrophils from patient blood in tubes EDTA. We are following the...

10 March 2024 4,156 3 View

Do my iPSCs have a normal morphology?

Hello everyone, I am starting to grow the SCTi003-A iPSC line for the first time and I am facing some drawbacks. Due to some problems with the first pass, I waited a bit longer than the 7-8 days...

22 February 2024 8,052 2 View

Why the best blocking solution to blot?

We already used milk: 5% to 1 hour, 7% to 2 hour, 10% to 1h, 5% overnight, but, , in all blockages it still marks the entire membrane, every run, there is a large band with no specific mark HELP

20 February 2024 7,346 0 View

Transcriptomic data sets with Staurosporine?

Hi, Does anyone know about a public transcriptomic dataset (RNAseq, microarray) of Arabidopsis (or other plants) treated with Staurosporine? I checked in GEO or SRA, but I couldn't find any....

11 February 2024 8,621 0 View

Is expert panel indispensable for pedagogical framework & scale development?

Hello everyone, Thank you for your time and patience in advance! I attempt to develop a pedagogical framework and a competence scale for my PhD project. I have developed the drafts of both. I...

06 May 2024 2,239 3 View

Compare the post modem perspective by Savikas career construction theory and the African perspective systems theory as framework for career choice?

compare the post modem perspective by Savikas career construction theory and the African perspective systems theory as framework for career choice.

10 April 2024 2,407 0 View

Where does conceptual framework/theoretical framework fall in a grounded theory (Strauss and Corbin)?

I am writing my research using the GT of Strauss and Corbin. However, typical research templates always have this Conceptual or Theoretical Frameworks.

09 April 2024 3,076 1 View

How do I revise the title of an article I on Reserach Gate?

Hi, My recent publication "Exploring cross-boundary collaboration for youth mental health in Sweden – a qualitative study using the integrative framework for collaborative governance". Is wrongly...

27 March 2024 3,153 2 View

Does anyone have access to literature supporting the idea that there are differences in energy demand across different levels of a building?

I'm working on the theoretical framework of my thesis and I can't find literature to support the idea that at different floor heights, there may be differences in energy demand. If someone can...

25 March 2024 4,998 1 View

Ecosystem policy roadmap how to creating the process?

Topic research : Ecosystem policy roadmapping Author : Leonardo Augusto de Vasconcelos Gomes About in my course subject innovation and technology management, My professor assign this research, I...

23 March 2024 8,707 1 View

What theoretical framework is appropriate for ensuring substantive representation of women parliamentarians?

I aim to explore the level of substantive representation among women parliamentarians, acknowledging the diversity within the female demographic and the prevalent male dominance within political...

22 March 2024 8,323 0 View

If the correlation is very less or negative in research framework in SPSS tool then what it indicates?

is there any mistake in the data or there is a probability of no connection between the variables? Kindly help me with the interpretation. Also, explain me the R square as well. I have attached...

18 March 2024 8,311 3 View

Is there a theory about the study of AI?

theoretical framework

10 March 2024 2,091 3 View

AIC under Bayesian framework?

Why we can not apply AIC under Bayesian framework. The AIC is based on the deviance and this latter is based on the likelihood. Now, if the likelihood function is available in closed-form and can...

09 March 2024 510 3 View