Does anyone have E. coli strain MDS42 ΔrecA trfA Blue?

More Alexandra Johnson's questions See All

Keeping cover slips from sliding around when growing cells on top of them?

I'm growing RAW 264.7 cells on poly-l-lysine coated cover slips in 6 well plates, infecting them with bacteria, then doing May Grunwald Giemsa staining on them. Following this, they're being...

09 October 2017 1,400 4 View

Have any protocols or tips for biparental mating of E. coli with gram positive organisms as the recipient?

I'm going to attempt a biparental mating of E. coli S17-1λpir (donor) with a few Enterococcus faecalis isolates (recipient). I've come across some publications that mention the RK2/RP4 transfer...

03 April 2017 2,837 1 View

Feedback defines the constitution of an organism?

“Here is a thought experiment. Let's place Rodolpho Llinas's jarred-brain on top of a body (Fig. 1). I bet Llinas would argue that his jarred-brain retains its own consciousness, and the android...

11 August 2024 2,483 1 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

What are examples of AI for good projects a teacher can assign to students?

So I am organizing an AI seminar. What are possible AI projects in the AI for good spirit? something the students can do and have an impact?

08 August 2024 9,437 4 View

Self-Organizing Superorganisms—as envisaged by Nenad Sestan (2018)?

The rate of glucose consumption by the neocortex is reduced by over 80% during anesthesia (Sibson et al. 1998), which disables the synapses (Richards 2002) that are inundated by glial tissue (Engl...

08 August 2024 3,118 0 View

How to design human-centered classroom in the age of A.I.?

08 August 2024 347 5 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do experts have journals in the field of artificial intelligence and big data that are not indexed by SCI or EI?

05 August 2024 8,836 2 View

Measuring the Intelligence of a Species?

Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...

05 August 2024 1,238 2 View

Riki P Tailor

Dear Alexandrasir, I think you should to contact Clinical & Laboratory Standards Institute. Find out more about this institute on internet.

Alexandra Johnson

Do you have any specific reason you think they would have it? I looked them up and I couldn't even find out if they had live bacterial strains or not, and I believe this specific cloning strain is pretty obscure.

Ok . i will contect here in india with microbiologist then i will inform you sir. I will inform you in one week.