Hello, check our papers, specially the last one.

Best Regards

Effects of low-frequency noise on cardiac collagen and cardiomyocyte ultrastructure: an immunohistochemical and electron microscopy study.

Antunes E, Borrecho G, Oliveira P, Alves de Matos AP, Brito J, Águas A, Martins dos Santos J.

Int J Clin Exp Pathol. 2013 Oct 15;6(11):2333-41.

Immunohistochemical evaluation of cardiac connexin43 in rats exposed to low-frequency noise.

Antunes E, Borrecho G, Oliveira P, Brito J, Águas A, Martins dos Santos J.

Int J Clin Exp Pathol. 2013 Aug 15;6(9):1874-9. eCollection 2013.

Histomorphometric evaluation of the coronary artery vessels in rats submitted to industrial noise.

Antunes E, Oliveira P, Oliveira MJ, Brito J, Aguas A, Martins dos SJ.

Acta Cardiol. 2013 Jun;68(3):285-9.

Myocardial fibrosis in rats exposed to low frequency noise.

Antunes E, Oliveira P, Borrecho G, Oliveira MJ, Brito J, Aguas A, Martins dos SJ.

Acta Cardiol. 2013 Jun;68(3):241-5.

Thanks Pedro, will check it out.

Hello,

maybe you can try dosage of hydroxyproline, a modified amino acid correlate with collagen quantity. There's a lot of papers that you could find to do this by ELISA techniques or by HPLC techniques starting from frozen tissus.

I have actually done this with ELISA starting from skeletal muscle and it went well (just finishing a master in cellular biology so it's pretty easy, thrust me). You can chose the technique that suits you better depending of the means that you have.

Hi, Dr Wang,

You may want to look this:

http://www.histosearch.com/histonet/Jun08A/HistonetQuantificationforA.html

However, there is no standard method to quantitate fibrosis. Both picrosirius red or Masson's trichrome staining are semi-quantitative, and hard to measure intensity. Picrosirius red will show structure change (different types of collagen) in some degree. It really depends on what question you want to answer. Do you want to know fibrotic area or collagen structure change, or myofibroblast differentiation?

Hydroxyproline assay is standard assay for collagen deposition (there are commercial available color based kits). You can also use Sircol assay (not as good as hydroxyproline, but simple). Second Harmonic Generation microscopy is very specific for collagen if you can find place to do it. You can use IHC to identify different collagen (Type I and Type III, in most case). There are some other extracellular matrix proteins that can be used for fibrosis marker, such as fibronectin-EDA, tenasin C. There are some other markers for fibrosis may be more tissue specific.

Hope my answers will help.

Hi, check this article it may be of help..

You can use ImageJ,,the results will be percentage of area,,

Thanks guys for your helpful suggestion! Read some papers to "score" the picrosirius red staining with Image J, did anyone do that? How? Could not find any paper mentioning how they scored it. 

Would be greatly appreciated!

Cheers