I could not find any method to prepare chitin azure. Before, Sigma was providing crab shell chitin for chitinase activity but now they have discontinued that product? I have to perform an in vitro test for chitinase activity.
If you wish to used Chitin azure from Sigma, it is still available and its catalog number is C3020. Its available in both 100mg and 1 g pack.
yes i know its present but i could not find any method to prepare chitin azure as chitin from crab shell had a specific method to prepare for analysis.
There are two papers which are referred world wide for making azure chitin .
1. Hackman R. H. and Goldberg M. (1964) New substrates
for use with chitinases. Analyt. Biochem. 8, 397-401.
2. J Microbiol Methods. 2004 Feb;56(2):213-9.
Colloidal chitin stained with Remazol Brilliant Blue R, a useful substrate to select chitinolytic microorganisms and to evaluate chitinases.
I am trying to get these papers for you. In case you can get them from some where you can also try.
Good luck
Thankyou i will see the mentioned papers but other than these if you could find any method in your university etc please let me know.
Anwar i recieved one of the papers and you can take that as ur benchmark for the preparation of azure chitin.... Good luck for your research :)
Dear Awan
I am sorry if i am too late to answer. There is a paper about chitin collodial. You can see the paper name below. I hope it will be helpful. The paper is;
MURTHY N. and Bleakley B., Simplified method of preparing colloidal chitin used for screening of chitinase-producing microorganisms, Current Microbiology, 57(5), 503-507, (2008).
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