Dear Shaima,

the response depends on the developmental stage of the oocytes. Give me information about the stage and I try being helpful.

Best regards

Pavel

I also agree with other doctors.

We need more information for the stage of oocytes.

Hi,

Collecting oocytes is simple. No need of superovulation.

Dispose the oocyte in M2 medium (containing cAMP to block meiosis and BSA). You dissect the ovary and will collect GV oocyte.

Then you wash them into a M2 medium drop and culture them under oil in M2 medium and they will get blocked in metaphase II.

I don't think you need to get them superovulated.