We recently bought a Sutter P-1000 electrode puller for pulling electrodes for patch clamping cardiac myocytes. However, we have been having a number of problems with the stability of our pipettes and recently have noticed that the jaw temperature is not decreasing when the air jets become active. More recently we also noticed some soot around the jaws. We have changed filaments on multiple occasions, and have also run the inbuilt diagnostics to no avail. We are using 2mm diameter glass and are running a 5 loop program to fabricate good pipettes.
Any advice would be more than welcome.
Thanks for your answer Jonas. On the P-1000 system there is a diagnostic tool to make sure this is flowing ok, and the temperature drops on the initial air jet before the pull (just not after the pull..). It may be that our heat settings are not high enough, so we are going to increase these, and hopefully that will improve the situation! Any further advice is always welcome!
Hi! also check that the driedrite granules in the tank on the back are still blue and did not turn purple. If they are purple, they are wet, and air will be as well, therefore not cooling as effectively. It is very simple to remove the tank and dry them in a conventional oven, Sutter has instructions on how to do that.
Regards,
Marco
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