Considering the use of this kit ( SQK-RBK004 ) for cost efficient sequencing of multiple bacterial genomes per flow cell. Any comments on its efficacy etc. would be welcome! Thanks!
The Rapid Barcoding Kit is a transposase based adapter ligation kit which is optimized for read lengths of 30 kb or more. However, the throughput of this kit is lower than the ligation sequencing kit. Moreover, no third party expensive reagents are required for this kit and the protocol is quite easy to perform. If your sequencing depth requirement is low then you can use this kit with the Flongle flow cell to make it even more cost effective. For making nanopore sequencing cost effective you need to multiplex as much sample as possible so that the cost of per sample goes down.