I am planning to measure the iron content (Fe2+, Fe3+) in macrophages with ICP-MS. Does someone have a protocol for this?
What kind of lysis agent should be used?
Thanks.
Digestion in nitric acid is suitable for this. But the instrument should ideally be run in kinetic energy discrimination (KED) mode, which helps in dealing with the argon-oxide interference.
ICP-MS alone will not give you speciation. You mention Fe+2, Fe+3. Do you mean measure both or measure total iron? Dr. Carter's recommendation of nitric acid will only give you total iron. To speciate you'll need to separate the two valence states prior to ICP-MS analysis, using ion chromatography or SPE.
Mr. Ho,
You can pay attention to: file:///C:/Users/CALLSHOP06/Downloads/11372_Appendix5TRACEprocedureformultielementanalysis.pdf
Thanks for the comments everyone.
Sorry, I misspoke - i am looking for total iron.
Bojidarka - I can't seem to access the file that you directed me to...
Sample perpetration must be using (HNO3 + H2O2) and use Helium gas in kinetic energy discrimination (KED) mode.
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