Looking for tips to optimise the IHC staining of PAX-2
Dear all, does any of you know free tools for conducting sentiment analysis in Italian that can take into account regional varieties too (as, for example, letting modifying the vocabulary)?
24 June 2021 2,959 2 View
I am investigating the efficacy of Gestalt therapy with adolescents engaging in self-harm, using a single case experimental design. I have administered some tools to measure the level of...
01 January 1970 6,056 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Does matter flow through ecosystems and energy is recycled and how does the flow of matter and energy compare in the living world?
03 August 2024 607 2 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...
02 August 2024 8,572 0 View
How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?
01 August 2024 7,061 2 View
As the construction industry advances, the integration of Project Data Analytics and Artificial Intelligence (AI) is becoming increasingly crucial in project management. These technologies are...
31 July 2024 6,484 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
