Does anyone know a company that does Pyrosequencing to determine mitochondrial DNA mutation heteroplasmy?

We are looking to genotype our mice. Our mice have a mutation in their mitochondrial DNA and we want to know the level of heteroplasmy i.e. % of mutation in their mitochondrial DNA. So we cannot...

09 November 2020 4,246 3 View

Low yield with HCl in chalcone synthesis: Why?

Hello, I can't understand why a low yield is obtained with the use of HCl in aldol condensation in acid catalysis for the synthesis of hydroxychalcone.

28 July 2020 7,853 5 View

Why do I get the same model fit indices for bollen-stine bootstrap and ML in AMOS?

Hi Guys, I would highly appreciate some help/input regarding boolen-stine bootstraping in AMOS. Given that my data violated the multivariate normality assumption I have opted to run bollen-stine...

12 May 2020 2,391 3 View

Positive Zeta Potential NPs dispersed in water?

Hello guys I am looking for positive zeta potential colloidal particles dispersed in water which I can order online. pH around 6 to 7. The size its from 300 nm until 700 nm. Thanks a lot for...

28 March 2020 6,869 3 View

Primers have 21&18 dna bases, tm of 43.9 & 45.1 and a GC content of 23.8% & 33.3% which is < 40 Does it affect varying annealing temperature?

our results showed varying temperatures and were aiming to get the optimum annealing temperature for the primer.

11 May 2019 7,254 3 View

Does anyone have experience with antibodies from Invitrogen (Flow Cytometry)?

28 April 2019 2,718 2 View

Low signal - Circadian luciferase assay in cell culture?

Hi, we are running circadian luciferase assays in cell lines (u2os and 3t3) driven by two different circadian promoters. We're reading the assay in a TECAN m200 luminometer using a black clear...

01 November 2018 7,642 1 View

Do mice have a dominant eye?

Do mice naturally have one eye that is more dominant than the other? The majority of people are right-eye dominant, and I was wondering if this could also be the case in mice.

08 May 2018 7,488 1 View

What will be the predisposing factor for the direct person-to-person transmission of zika virus?

So far, 13 cases of direct person-to-person transmission of zika virus have been documented, and the only predisposing factor appears to be a high viral load of patients who are critically ill.

22 July 2017 8,876 3 View

Tesr

Test

07 February 2017 6,051 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View