Does anyone have a simple protocol for cell isolation from plucked hair?

21 August 2013 0 4K Report

I want to obtain a cell in metaphase from a culture of plucked hair

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Where can we get few mm diameter polished circular glass plates?

who retails few mm diameter polished circular glass plates ? thickness 0.1 to 1 mm diameter 1 to 3 mm ordinary or fused silica glass polished on both sides and the edge

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Looking for Validators?

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Why would a primer produce a product for PCR but does not work for Sanger sequencing?

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Please, could you delete that page of Pangou ?

Dear Editor, I'm shocked that you publicized some papers that are fake here see https://www.researchgate.net/scientific-contributions/Serge-Valentin-Pangou-2003482514 See also the pdf attached...

26 March 2024 7,871 2 View

Could you recommend a good ointment base for wound healing?

Could you recommend a ointment base for adding potential active compounds and evaluate the benefits for wound healing? A database for ointment formulations? A basic procedure to prepare elemental...

02 October 2023 6,344 0 View

How can I publish an article?

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27 September 2023 4,821 2 View

"Group Representations, Algebraic Geometry and Topology in Quantum Information and (Epi)Genetics, how are they related?

We launched a special issue about the topic in the title https://www.mdpi.com/journal/quantumrep/special_issues/6YGGQI4308 Please send comments and possibly a submitted contribution. Thanks in...

06 September 2023 7,678 0 View

Temperature sensors - low cost?

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16 February 2023 7,280 1 View

BLDC air gap radial flux density calculation problem ?

Hello everyone, I am trying to estimate the air gap flux density in a surface mounted BLDC motor. To do so, I am refering to the book Brushless Permanant Magnet Motor Design second edition. The...

19 December 2022 1,081 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

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06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

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Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

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04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

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04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

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04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

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04 August 2024 7,282 2 View