What test should I use?

15 February 2021 911 5 View

How remove endotoxins from DEAE-Sepharose?

Hi to everyone! I have to validate endotoxins removal from DEAE-Sepharose I use for the purification of my hormone. The resin undergoes to a sanitization protocol with NaOH. To demonstrate the...

29 October 2020 9,141 4 View

How can I roughly estimate the current denisty in an FPGA?

Hello, I am currently working on the estimation of the electromigration of an FPGA. We just performed the construction analysis so we know all the values of wire width and height for each metal...

22 June 2020 2,743 3 View

What type of proteasome inhibitors is better to use in tissue lysate?

Hi all. I am looking for a proteasoma inhibitor to use in my tissue lysate. I found MG132; lactacystin and PR39. Anyone know which of these is more effective to use in a tissue lysate?, Do I need...

16 April 2020 4,088 3 View

What is the (average) DNA yield (ng) from one single nematode?

Do you know where I can found published information about DNA quantity (in ng) from one single nematode? Any publications/books to suggest me? I Know it seems a simple and basic information, but...

31 March 2020 6,710 1 View

Can I use Arrhenius Relationship to determine Accelerated factor , when test temperature is lower than use temperature?

Hello, I have a question regarding the acceleration factor. We know that the lifetime (TF) of a component can be expressed as: (1) TF=A0exp(Ea/(kT)) where A0 is a constant (component...

01 March 2020 6,635 6 View

Does anyone have experience with fMLP as a neutrophil chemoattractant in a transmigration assay?

I am planning to perform a transmigration assay (transwell plate) in which are involved neutrophils , therefore I will use fMLP as a chemoattractant. I did find some papers that describe "The...

19 February 2020 5,855 3 View

Where to publish a short descriptive note about a novel behaviour?

I have found a novel behaviour in an insect and I would like to publish it, but I do not know where to publish something that is just descriptive (with photos and videos) and does not includes any...

13 June 2019 401 1 View

Is there a pigment that can be used to dye PMMA in anisole?

I am looking for an organic dye, soluble in anisole to be able to get coloured PMMA films

30 April 2019 812 1 View

Simultaneous fixation, permeabilization and Phalloidin staining of adherent cells?

Dear all, does anybody have experience with the simultaneous fixation, permeabilization and Phalloidin staining of adherent cells (fibroblast or endothelial cells/cell lines)? Many companies...

25 June 2018 2,381 4 View

Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View

CRISPR/Cas9 oligo design ideas and suggestions?

I need to design new oligonucleotides that can be used for gRNa/Cas9. The oligonucleotides need to target a gene from melangoster drosophila. Where do I start? How to find the gene to knock...

15 December 2020 4,729 3 View

How does the Laser Power Meter measure the light? Is it the power of light per unit area?

I am doing optogenetics on Drosophila larvae. I need to use the Blue light and I have to measure the light intensity. For this purpose, I am using "Sanwa Laser Power Meter LP1". The display shows...

11 August 2020 8,696 3 View

Does using ethanol for de-waxing drosophila heads before dissection interfere with future protein extraction or fly brain imaging?

I have heard that dipping fly heads in 70% ethanol before placing in cold PBS for dissection can help strip the heads of wax and allow them to stay submerged in the PBS during dissection. I was...

10 August 2020 4,373 2 View

Protocol for combining FISH with IHC?

Hello, we are developing a protocol for combining FISH and IHC on Drosophila embryonic brain, using FISH to localize mRNA and IHC (2 antibodies) to mark neuropil and neural tracks. We thought...

02 March 2020 3,896 1 View

Protocol for combining FISH with IHC?

Hello, we are developing a protocol for combining FISH and IHC on Drosophila embryonic brain, using FISH to localize mRNA and IHC (2 antibodies) to mark neuropil and neural tracks. We thought...

29 February 2020 3,291 3 View

What chemical or substance can I use as an alternative to propionic acid for breeding Drosophila?

Hello. I am looking for a cheaper alternative to propionic acid which I can use for breeding Drosophila. I am currently teaching Evolutionary Biology and I would like my students to conduct...

11 December 2019 6,313 2 View

Another solvent different to DMSO and Ethanol for picrotoxin?

Im working with drosophila, and i want to avoid those solvent because the influence the may have in memory.

11 December 2019 3,638 2 View

Best way to quantify tumor size/area from confocal images?

I have been imaging many z stacks of Drosophila mid/hindgut tumors using a leica confocal microscope. I'm wondering what the best way to quantify these tumors would be. They are GFP+. I know both...

11 December 2019 7,260 3 View

Best way to quantify tumor size/area from confocal images?

I have been imaging many z stacks of Drosophila mid/hindgut tumors using a leica confocal microscope. I'm wondering what the best way to quantify these tumors would be. They are GFP+. I know both...

02 December 2019 4,584 3 View