I intend to incubate cells in the media for a few days post DNase treatment.
Dear Research Gate Members, We all have experience regarding publications of research/review articles in various journals. Some of the journals review the articles in 4-6 weeks and some takes...
01 October 2020 7,201 1 View
I am currently working on collecting literature based on the available treatment technologies for industrial effluents. There are vast variety of methods physical, biological and Advanced...
23 September 2020 2,314 4 View
Flood frequency analysis is mainly based on the assumption of stationarity of the data; however, in recent years, this assumption has been open to doubt. So before carrying out the FFA study, what...
07 September 2020 2,586 3 View
According to the power law i=av^b, for a material (reduced graphene oxide) to be capacitive the value of b=1 whereas b=0.5 for battery like behaviour. However, the CV shows a quasi rectangular...
26 February 2020 4,592 4 View
I have injected 5 samples of lowest possible concentrations. I have taken the std deviation of area and slope of the equation obtained for the regression line of calibration to calculate LOD &...
19 January 2020 4,730 7 View
I would like to perform linkage disequilibrium test for a number of SNPs. I am absolutely new to this field and would like to know what input file should be taken for my SNPs. I also would like to...
11 December 2019 8,416 2 View
Current researches to cure Parkinson's disease deal with the formation of the dopaminergic neurons from the Skin fibroblasts but even after the formation of neurons, is their administration to the...
25 August 2019 3,585 2 View
Hey, Imagine a scenario, I am required to train machine learning model on ABC data-set. There are few pre-trained models trained on same ABC data-set. Can I use these pre-trained models in my...
26 January 2019 2,057 8 View
HI, I am screening compounds against my synthetase enzyme with thermal shift assay. I have encountered a drug which is showing a shift of around 20degrees from the Tm of my protein. I am using 2X...
22 January 2019 4,457 1 View
Can peripheral blood be used to check neuroinflammation or microglial activation? or Do we have any markers in the peripheral blood which can detected either by ELISA or flow cytometry and give us...
17 August 2018 6,015 7 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Incompletely cross-linked pdms are difficult to remove.
01 March 2021 798 3 View
To maintain the proper crop plan most of the times there is not enough time to make the field ready for the next crop. In this particular context , can bio-decompser be a solution? What about...
16 February 2021 7,068 24 View
Hi there, I'm isolating neurons from PND1 guinea pigs and have noticed a bit of debris in the early days (this is day 4 plated) and a lot of variability between plates. Current protocol is...
14 February 2021 3,692 4 View
14 February 2021 4,194 3 View
I am trying to do some MD simulations of a protein with zinc ions. I am trying to use a charmm forcefield (I have tried 2017 and 2021) and Gromacs. I get an error when doing pdb2gmx Residue 'ZN'...
12 February 2021 9,367 2 View
I am trying to run a molecular dynamics simulation for a ligand that contains a "SEP" residue. also, I am preparing my files using Charmm-GUI using charmm36 ff, but it says that it will rename my...
31 January 2021 6,321 1 View
30 January 2021 4,684 4 View
I am trying to perform homology modeling using the swiss-model but I have found that the swiss-model gives the same number of residues as that of the template. Whereas my sequence has more...
21 January 2021 5,568 3 View
I am looking some resist strippers which can easily remove metal based polymer residue after post dry etch process. I have some candidates below but not sure if they are compatible with doped...
21 January 2021 3,008 3 View