Does anyone have a protocol for co-IP of histones in tobacco?

01 January 2014 0 2K Report

I tried IP protocol with dynabeads, and I know that IP worked, because of my GFP tagged control, but for some reason I can't detect histones on my blots (for western I am using abcam anti-histone H3 antibody) I am starting immunoprecipitation with 1 ml of tissue, maybe that's too low? I would appreciate any advise, suggestion etc.

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