20 May 2019 1 3K Report

I'm currently searching for natural killer cell cytotoxicity tests that are not based on flow cytometry but on calcein release measured in the supernatant. Can anyone help with protocols?

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Hello everyone, I'm working with a PA-1 ovarian cancer cell line xenograft model in female nude mice. My cells are at 372 passages, the cell repository bank themselves gave at 352th passage....

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Is there a study on the LCA of Kapton/Polyimide available?

As Kapton is one of the predominantly used substrates in printed electronics applications I was wondering if there is a study on the LCA of Kapton/Polyimide available? At least considering the raw...

21 May 2024 7,402 0 View

Why can't the lactate dehydrogenase test be run in the majority of clinical chemistry tests in the use of lithium heparinized plasma tubes?

According to the study "The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory", most clinical chemistry and immunoassay tests can be performed...

09 April 2024 6,395 1 View

What are strategic challenges for insurance companies against the backdrop of digitalization?

What are the biggest strategic challenges that insurance companies are facing as a result of digitalisation?

19 November 2023 2,728 5 View

How to achieve highest aeration in small (10ml) culture volumes?

Hello everybody, For my research I have to optimize the growth in liquid cultures of a bacterium for which oxygen availability is very important. Long story short, there will be other factors to...

22 June 2023 167 2 View

IHC wash details?

Hi, I was wondering if it's possible to decrease non-specific background in IHC staining by using 37C washes instead of room temperature washes. Thanks.

19 June 2023 3,538 1 View

I am running a mediation analysis using PROCESS macro for SPSS. How should I interpret a higher estimate for the direct than the indirect effect?

In my model the indirect effect is significant and the direct effect is not significant. However, the estimate of the direct effect is much higher than the one of the indirect path. Should I also...

05 June 2023 387 11 View

Why is Tris-HCl much more popular than PBS/HEPES for cell lysis buffers?

Hello! I am preparing a protocol for cell lysis of tissue samples. I want native conformation of as many proteins as possible and would therefore like to have good pH control. In most protocols,...

24 May 2023 3,399 6 View

Optimal buffer for total native protein extract from tissue?

Hello! I am working on a protocol to extract as many native proteins as possible from brain tissue. The end goal is to use the supernatant of the lysate in immunoprecipitation against some...

08 May 2023 7,943 1 View

How do you analyze the cell cycle with flowjo? from a .c6 file?

When i try to analyze the cell cycle in flow jo from a bd accuri c6 file i get an error. I followed the instructions in the tutorial in youtube. I tried to increase the number of events but the...

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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How to improve plaques in Double Layer Agar Assay?

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Why activated CAR-Jurkat cell could not kill targets?

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The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

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Are air moisture harvesting technologies effective in combating desertification?

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How to determine positive-stained cells in FACS? Use isotype or unstained control?

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How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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Why do we equate male and female arousal?

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How to use Density Functional Theory to calculate carrier mobilities of solid system?

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