Is there a typical range in the power spectrum that each person has during a rest measurement? Moreover, are there differences between men and women?

Is there a typical range in the power spectrum that each person has in the individual brain areas (19 electrodes, 10-20 system) and frequency bands (theta, alpha, beta gamma) during a rest...

13 January 2021 5,660 3 View

How can I find electricity generation data of small to medium scale PV panels and Wind turbines?

I am using model predictive control to simulate market market participation of a Virtual Power Plant in the north of the Netherlands. The power plant portfolio consists of local (geographical...

21 September 2020 4,409 11 View

Does anyone have experience with nattokinase, or another way to dissolve fibrogen?

We want to extract RNA from a 3D TC and nattokinase was suggested, but i am not sure whats the best supplier - dissolving technique, and amount and incubation time to add to the 3D TC ?

01 September 2020 7,424 3 View

How to separate monocytes by plastic adhesion?

07 July 2020 5,883 10 View

Does anyone know what kind of insect this is?

The flower was found from a Hydrangea species in my garden. These little bugs were found hiding within the flower. Do you know what they are?

11 August 2019 3,037 8 View

Can I calculate the coherence of two EEG signals on a given frequency band or do I have to calculate them over the entire frequency range?

I would like to know whether I first have to calculate the coherence between two signals over the entire frequency spectrum in matlab and summarize the specific frequency bands from these values...

09 May 2019 9,695 2 View

Cell line recommendations for transfection of plasmid vector with human insert?

I have constructed a plasmid with a human DNA insert (5kb) that has the luciferase reporter gene. I will be looking to transfect this plasmid into mammalian cells and run a luciferase assay to...

25 January 2019 3,991 3 View

How to optimise PCR for linearising 4.2kb plasmid?

I'm trying to linearise a plasmid that I will use as my vector for NEBuilder HiFi DNA Assembly. I have designed primers for linearising the plasmid that I am now testing. There are shorter bands...

04 December 2018 8,935 3 View

Has anyone used a Vglu2 anti mouse antibody on mouse brain?

18 October 2016 3,905 3 View

I want to coat a 96-well plate with Heparan Sulfate. Is there a good alternative for Heparin/GAG Binding Plates which are hard to get and expensive?

I want to do a cell adhesion assay on a 96-well Heparan Sulfate coated plate. The Heparan Sulfate should bind as naturally as possible without much changing of its conformation. I could only find...

20 September 2016 6,370 13 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View