Hi Bernardo,

find attached a paper publised by our team on this particular method , if you have any supplemental questions please feel free. We adapted this method for catalase and SOD also which you can find in the second papaer.

Cheers Sanders

Dear Shahkrokh thank you very much but that is pretty much the normal methods for enzymatic activities that I've used normally. I'm interested in papers focusing miniaturized microplate methods only for anti-oxidant enzymes.

Hi Bernardo.. I follow a protocol from the following paper..but it is only for antioxidants (GSH and Ascorbate)..not for the enzymes... Please see If this also helps you..

sorry..for got the reference..

Queval, G. and Noctor, G. (2007) A plate-reader method for the measurement of NAD, NADP, glutathione and ascorbate in tissue extracts. Application to redox profiling during Arabidopsis rosette development. Anal. Biochem. 363, 58–69.

Dear Sunil

Thank you very much I have that paper I'm after one for anti-oxidant enzymes in a microplate specifically due to the high amount of samples that I have

The following article may be of interest to your purpose: Kelly M Gillespie, June M Chae & Elizabeth A Ainsworth (2007) Rapid measurement of total antioxidant capacity in plants. Nature Protocols 2: 867-870.

Thank you Rafael but I'm looking for a microplate method for anti-oxidant enzymes.. But thanks anyway!

Hi Bernardo,

find attached a paper publised by our team on this particular method , if you have any supplemental questions please feel free. We adapted this method for catalase and SOD also which you can find in the second papaer.

Cheers Sanders

please find the second paper attached.

hope it will help and best of luck for your essays

Sanders

Thank you very much Sanders! It was exactly this that I was looking for. Cheers!

Useful information for me, too. Thanks a lot!

yes agree with Hua this was very useful information and good reference

Works of Sanders were very usefull for me too.

Thanks a lot.

Hi Sanders

Your papers are really helpful and easing off the work load of taking cuvette readings.

Thanks