Does anyone have a good protocol for NE-CE preparation from THP1 cells?

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Navneet Ammal Kaidery

I used NE-PER kit from Thermo Fisher (78833). It consistently gave me good results. (I added an additional wash step with cold PBS before solubilizing the nuclear pellet).

Atul Udgata

Thanks Navneet, will give it a try !

Mohammed Hakim Jafferali

I have protocol that works for Nuclear pellet isolation from cell cultures. Do you have access to swinging bucket ultra centrifuge that goes upto 32000 rpm. if so let me know i can give my protocol.