Mohammed Hakim Jafferali

3 Questions 24 Answers 0 Followers

Questions related from Mohammed Hakim Jafferali

QPCR----NTC amplification in GOI but not in reference gene ?

Hi. I over-expressed plasmids in the cell culture and collected RNA then reverse transcribed to cDNA with RT negative controls. Then I performed qPCR with SYBR green system. I observe...

07 July 2018 10,009 8 View

U6 or hUBC promoter plasmid for ShRNA plasmid?

Hi, I have designed ShRNA oligos and will be ligating in plasmid. I want know if my shRNA will work if I have hUBC promoter in my plasmid? Or do i need to have U6 promoter.

03 March 2016 3,613 2 View

Can some one suggest immortal mouse muscle cell lines other than c2c12?

I have c2c12 cell lines which on serum deprivation or changing to horse serum, the cells differentiate into myotubes. Now am looking for other immortal muscle cell lines like c2c12. It can be...

02 February 2015 3,578 7 View