mRNA detection, whole mount, imaging techniques, zebrafish
I am aware of theseArticle Whole-mount single molecule FISH method for zebrafish embryo
Article Multiplex Fluorescent In Situ Hybridization in Zebrafish Emb...
And wonder whether this one would work with some minimal tweaking...Article A safer, urea-based in situ hybridization method improves de...
The third one looks very promising.
Best,
D
Great! Thanks Daniel
This is referring to tol2 gateway cloning and gene expression levels. Thanks in advance for your replies.
31 December 2014 681 7 View
zebrafish transgenesis, Gal4/UAS expression system
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Gal4 zebrafish transgenesis. How to identify germline carriers.
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"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
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What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
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How to calculate the molar proportions of the oxides from the XRF analyses?
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Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
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How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
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