AMBER tleap solvatebox always undervalues the density, why?

I've been searching for an answer to this question for years, and I'm shocked I haven't found any discussion on any forums. Here is an example to provide more details: I am preparing a 450...

20 January 2021 7,561 2 View

Are there available scales or questionnaires to measure change management?

Seeking to connect with authors who may want to share their scales or questionnaires to measure change management.

27 September 2020 374 6 View

How do I combine two variables into one in SPSS?

I have a data set with information on BMI which is derived either from self-reported (variable 1) or measured weight (variable 2). Each subject has information on BMI in one of these variables,...

07 October 2019 431 3 View

How to export ANSYS Static Structural results to a format that Paraview can read?

My ANSYS model gives me .rst and .dat output files and Paraview gives me errors when I open them. How to get ANSYS to export a format that Paraview can successfully read? Thank you :)

18 March 2019 1,481 1 View

Statistical differences between SPM and FreeSurfer?

Hi, Does anyone know what are the statistical differences between SPM and FreeSurfer? Thanks!

19 September 2017 3,360 5 View

How to remove protein from DNA mini prep?

17 October 2016 3,050 6 View

Is plasma treatment comparable to corona treatment to prepare a thermoplastic surface for bonding with an adhesive?

30 August 2016 4,951 4 View

How effective is the restriction digestion step for TRF telomere length measurement?

I read that after HinfI and RsaI digestion the non-telomeric fragments are typically

01 March 2015 5,923 5 View

Can anyone suggest a transcription service that does Spanish interviews?

Looking for a Spanish translation service, preferably US-based.

02 February 2015 5,434 5 View

Is there a way to translate amino acid sequences into nucleotide sequences?

I am interested in looking for evidence of positive selection across domains for a group of genes I am working on. I have collected nucleotide coding sequences for each gene from NCBI. These...

30 May 2014 4,126 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View