Can lenti-virus plasmid be integrated into the genome of 293T cells?

293T cells can be used as producer cells of lentivirus particles to infect other target cells(eg, in order to over-express or silence a gene ), but I was wondering if the virus in turn to infect...

10 December 2019 4,763 2 View

How to get the SNP flanking sequence(for Prunus mume, Japanese apricot)?

I already get SNP candidates for our target traits, then I would like use Real-time PCR based genotyping platform to check F1 population. For establishing the marker, the SNP flanking sequences...

09 November 2019 331 3 View

How do I stain bacteria and use confocal microscope to observe them?

08 June 2018 522 3 View

Methods for ApoB48 western blotfrom density gradient fractions?

I have cultured primary enterocytes with lipids. After then I collected the medium and cells' microsome lumenal proteins for NaBr gradient (d=1.006~1.10), and each fraction (1ml) was collected...

30 July 2017 7,697 5 View

Methods for ApoB48 western blotfrom density gradient fractions?

I have cultured primary enterocytes with lipids. After then I collected the medium and cells' microsome lumenal proteins for NaBr gradient (d=1.006~1.10), and each fraction (1ml) was collected...

06 July 2017 7,805 6 View

How to properly compute phylogeny for closely related species/strains?

04 May 2017 4,661 2 View

How to use VASP to calculate the optical properties of graphene?

I want to know the dielectric constant of CNT under any bias voltage by using VASP.

21 August 2016 962 2 View

No title

The culture medium which ATCC suggests: RPMI 1640 with 20% FBS does not work. Need help.

27 April 2016 359 2 View

Are there diseases related to the concentration of trypsin?

I'm looking for any medical conditions that trypsin concentration significantly increases in the disease state. Any information on the concentration of trypsin in normal environment versus disease...

02 February 2016 9,922 3 View

Can anyone tell me your daily diet for Bongo Tragelaphus eurycerus?

15 March 2015 3,465 4 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View