Calculate pH after addition of base?

04 February 2021 2,232 4 View

Western blot with very acidic proteins, any suggestions?

I am trying to do a Western Blot to detect acidic proteins (~30kDa) that have a PI of ~3.5, due to the presence of Asp and Glu repeats. The proteins are about 80% pure. After I ran them on...

24 January 2021 7,226 3 View

How does one create IFORM contours?

Hello: Can anyone assist with plotting IFORM contours. I have been trying to plot contours using Hs and Tp as variables. The marginal distribution is a GPD-Kernel density and the conditional...

12 December 2020 4,355 3 View

How can I transfer heat between groups in Gromacs?

Is it possible to transfer heat from one group to another using simulated aneeling technique in the Gromacs software? I would like to transfer heat from a gold nanoparticle (group 1 from 310 to...

20 November 2020 9,062 3 View

Would ER NiCrMo-3 be the best option for weld overlay of carbon and low alloy steels?

Alloy 625 is the most popular alloy for weld overlay. Without considering the price factor, would NiCrMo-3 be the best technical option for weld overlay of API 5L and low alloy steels, in general,...

24 August 2020 2,478 4 View

Dose-response on non-Michaelian response enzymes, how?

Hello For typical dose-response assays, our lab usually uses steady state intervals for defining the difference between control and tested compound. For those assays, we typically use the angular...

23 July 2020 8,662 2 View

What is the best way to graph an SVM classification in R or Python?

I'm trying to find a way to graph a classification made by the support vector machine algorithm. Something like a PCA or LDA chart would be excellent. Any help will be welcome.

26 April 2020 4,588 1 View

Gene therapy with transgenics viruses Covid-19?

Viral load and symptomatic picture Is there any relationship between the viral load of exposure and contamination, in number of viruses and contamination processes, and the symptomatic picture in...

08 April 2020 6,349 2 View

Is it allowed to publish part of a journal paper(under review) as a conference paper?

Is it allowed to publish part of a journal paper under review as a conference paper? I have submitted a full research paper to a journal. I'm waiting for the review answer and I want to submit...

01 April 2020 430 6 View

Do KG-1a human macrophages need to be activated in some way?

Hello everyone. I was wondering if you can help me clarify this question. I am seeking to perform infection assays with bacteria, using this human cell line. Research articles regarding infection...

24 March 2020 9,884 3 View

Has anyone seen this perivascular black stuff in rat brains?

This photo is from a reportedly healthy Long-Evans rat. Several of these animals have similar findings. No other sign of illness. Has anyone out there seen this?

19 November 2020 8,108 4 View

How does Anti-retroviral therapy effect cryptococcal meningitis?

I have read that early administration of ART in cases effected with cryptococcal meningitis lead to a higher level of mortality but unfortunately could not get a clear understanding about the...

31 December 2018 7,040 0 View

Which equipment do I need to use to perform intracerebral meningitis infection effectively? And could you tell me some tips for the injections?

Hello! I plan to check the protective effect of the adoptive transfer of some memory CD8 T-cells against the LCMV infection. So I think to establish LCMV meningitis model and to see whether the...

11 December 2018 8,189 3 View

Persistent Coxsackievirus B in the CSF (help)?

Clinically the patient had the encephalitis/meningitis 9 months ago but PCR from LP has provided the reason for the continued symptoms. Anyone have expert knowledge in what can be done aside from...

07 August 2018 9,454 8 View

Does anyone have knowledge/evidence of the presence or absence of vapor collections in the domes of in situ ventriculoperitoneal shunts or reservoirs?

My project is to study the possibility of evaluating intra-cranial pressure for patients with ventriculoperitoneal shunts or reservoirs.

03 April 2018 6,792 2 View

IVIG and aseptic meningitis, is there any relationship between them?

Nowadays IVIG is prescribed in many rheumatologic and immunologic diseases like GBS and SLE. In some texts, aseptic meningitis is one of the rare side effect of IVIG... I want to collect some new...

02 March 2018 7,108 4 View

What are the possible problems encountered with carotid shunts and their possible solutions?

What are the problems encountered with carotid shunts that are used in carotid endarterectomy and their possible solutions?

31 December 2017 1,001 2 View

How food has been used as a medicine?

Dear RG colleagues, Some of us are suffering from disease including me where I removed my gallbladder by laparoscopic surgery, so I think it is best to depend on food like fresh vegetable and...

07 August 2017 8,696 16 View

What data logger can use to measure the photocurrent, photovoltage, shunt and serie resistance of solar cell?

What data logger can use to measure the photocurrent, photovoltage, shunt and serie resistance of solar cell?

05 June 2017 9,884 3 View

How many cases of complications of influenza A(H1N1) in the form of meningitis or menigoencephalitis in the world

How many cases of complications of influenza A(H1N1) in the form of meningitis or menigoencephalitis in the world

31 December 2015 1,736 4 View