I am working on engineered virus (which has linearized genome) with BAC (Bacterial Artificial Chromosomes), and struggling to get circularized DNA (episomal DNA) from infected cells. So far I have been trying using Hirt Lysis protocols and transfer the DNA into E.coli. However, I have never got complete genome after extracting the DNA from the bacteria. And now I would like to find any other alternative kits for dealing with this. Does any of you have experience with this? Could you give me suggestion what kits that I can use?

Best regards,