Vector
I am trying to label a membrane protein with MTSSL (a thio specific spin label). The Membrane protein is a single cysteine mutants at different positions for EPR measurements. The Protein is in...
03 April 2013 3,604 7 View
I want to make a figure which have a sequence and its structure Annotated on top of it. If somebody knows any software related to this then please let me know. I am not asking any structure...
09 October 2012 8,669 3 View
I am looking for a software with which I can represent my multiple sequence alignment results in a more concise representative block diagram, which could show the relative conservation of residues...
07 August 2012 9,400 6 View
Elsevier withdraws support for the research works act
01 February 2012 8,256 2 View
Can anybody suggest me a Journal related to human disease? I want to submit a paper which shows the structural basis of a rare human disease. I can go to JBC or JMB... but I wanted to send in a...
31 December 2011 6,216 7 View
HI, Can anybody suggest me a Journal related to human disease? I want to submit a paper which shows the structural basis of a rare human disease. I can go to JBC or JMB...but I wanted to send in...
31 December 2011 9,694 4 View
I am interested in obtaining a gene for a particular protein sequences (1000 amino acids) from genescript or from somewhere else. I am confused if i should go for a codon optimization for the...
31 December 2011 1,161 1 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
