Does anybody used GelRed instead of etidium bromide in an Eckhardt plasmid preparations?

10 October 2013 0 7K Report

I want to improve the in situ lysis preparation of plasmid DNA.

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How to confirm the site-directed mutagenesis result without performing NGS?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

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