Does anybody use meso plex MSD assays? What are your opinions?
Are they really more sensitive than regular, old ELISAs?
Hi, In the 6 well plate, when I am seeding the cells. Do I add 1ml of complete media to one of the wells for the control at this stage or do I wait unit I am treating the plates to do that? Also...
13 July 2024 9,783 1 View
I intend to study butterflies and moths diversity including their species richness and evenness for their conservation.
06 August 2023 8,308 7 View
I appreciate that the agreement (or otherwise) between findings/results in a study and other published literature, is usually found in a discussion chapter. However, are there circumstances in...
27 June 2023 7,700 3 View
The experiment involves a cancer cell line. So that I can normalize the experiment properly, I need DNA extraction from the sample, alongside with the RNA extraction results. Both extractions must...
13 May 2023 657 4 View
I need help with coding in R (Gapminder) Here is my code: > gapminder %>% + group_by(country, year = 2002)%>% + summarize(population > 10000000, meanlifeExp = mean(lifeExp))
10 November 2022 6,412 0 View
Hello everyone, I'm planning a study that involves treating mice with the PPAR gamma agonist rosiglitazone. It seems like I have a few options for administering Rosi, including gavage, ip...
19 July 2022 1,030 2 View
Yes, we could just express BirA from a plasmid, but we hope to eventually use this protocol in cells that are difficult to transfect. It would be more convenient to just toss some BirA into the...
05 April 2022 9,070 1 View
On three separate occasions I have encountered this mite, what seems to be the of same species, attached to the proboscis of Aedes aegypti and Culex erraticus species of mosquitoes. My guess is...
25 August 2021 1,197 7 View
Hi all I have conducted a study looking at different teaching styles used by teachers in two different studies. I have two Hypothesis: 1. Teaching styles will differ across the WERID (the...
15 August 2021 5,109 4 View
Most, if not all, publishers approach the acquisition of original work through the transfer of publication rights of the author to those of the publisher. Hence, the copyright is given to the...
07 March 2021 2,746 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
The Likert scale is a commonly used measurement tool in social science research to assess people's attitudes, opinions, or perceptions. The social science students still have difficulty selecting...
25 July 2024 7,535 1 View
It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...
24 July 2024 8,596 2 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
