MINOCYCLINE safe to use 3 months after expiry?

I plan to call my PCP tomorrow to refill it but I had some left over from a spinal infection (which reoccurs) so I took the initial dose today. Its 3 months past its expiry date. I've seen that it...

30 July 2020 9,983 7 View

Why arn't small MW proteins transferring?

25 August 2016 7,374 11 View

Does anyone regularly use a vaporizer to administer isoflurane for anesthetizing mice?

I'm trying to anticipate how much isoflurane I will be using in a year. I will be anesthetizing about 200 mice for about 10 minutes each mouse over the course of a year. Can anyone approximate how...

09 December 2015 2,501 2 View

Why am I getting a peak at the same temperature as my samples for my NEC?

I continually get peaks in my no enzyme control for real time PCR in my melt curve. Though, my Cts are ~7 cycles away. I have DNase treated my RNA twice, changed all reagents and decontaminated...

28 October 2015 6,334 2 View

Would you like to collaborate on a medical device regulatory book contracted through a reputable organization?

03 April 2015 8,554 8 View

Can anyone recommend a general anti fungal drug to add to a bacteria media to avoid contamination?

13 October 2014 152 10 View

Why is the supernatant from my pET expression system lysis step so viscous?

I have been expressing and lysing this construct for years, and today I got a thick gel instead of a nice supernatent. I recently started using Magic Media instead of IPTG induction, but this has...

05 September 2014 5,403 4 View

Which breast cancer cell lines do you find grow best in nude mice?

I have worked exclusively with triple negative MDA-MB-231. We are thinking about redoing projects using a different TNBC cell line or ER+ line such as BT20 or MCF7, respectively. The xenograft...

07 July 2014 8,830 6 View

Domo for decision-making?

I have seen several ads referring to DOMO as a better method for tracking data vs. Excel Spreadsheets because it has the capacity to inform decision-making. Has anyone seen a demo and/or used the...

06 July 2014 7,556 2 View

How long can fresh frozen tissue be stored at -80C?

I need to store fresh frozen tumor tissue from nude mice for western blotting, receptor binding assays, and immunohistochemistry. In our lab, we freeze tissue for western blots in RIPA buffer, in...

14 May 2014 1,481 9 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Online surveys for social sciences researchs: what are your opinions about this tool and how can we improve the research design to incorporate online?

Good morning everyone, Due to the pandemic, I conducted several online surveys. It is quite agile, not at all expensive, but there are several problems: the sample's representativeness and its...

21 February 2021 7,457 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View