Humic acid omnipotent is an integral part of organic matter playing an important role in the ecosystem. Unfortunately having a dark color it is quite difficult to work on using spec. methods.
Hi Raza Khan Janab; Go through the following links which may be useful to you. Regards
http://journals.lww.com/soilsci/Abstract/1997/03000/ANALYSIS_OF_CARBOXYL_GROUPS_IN_SOIL__HUMIC_ACIDS.4.aspx
http://pubs.acs.org/doi/abs/10.1021/ac00255a014
For UV/visible spectrophotometry, you can simply dilute the sample(carefully) in pure water. All of the wavelengths that you are interested in should give absorbance values of less than 1.5 (or 1 depending on the instrument). You can then correct the absorbance value according to the dilution factor. Here is a paper I recently published that includes UV/Visible measurements on humic rich swamp water samples (not isolated humic acids, but similarly dark color), you may find the method section and some of the references within useful.
Good luck!
http://aslo.net/lo/toc/vol_53/issue_3/0955.pdf
Hi John. Im very interest in your method. I did it for aquatic humic substances in Matanza Riachuelo River Now Im working on a paper with this
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