AS originally pointed out by Monod in 1949, handling microbial cultures is a fundamental methodology of microbiology and mastering different cultivation technique should be a of every micribiologist's craftmanship. If microorganisms grow as single dispersed cells and not as cell aggregates in culture media, a small volume can be taken of the broth culture and highly reproducible measurements can be obtained. The growth of bacterial cultures, despite the immense complexity of the phenomena to which it testifies, generally obeys relatively simple laws, which make it possible to define certain quntitative characteristics of the growth cycle. For more details consult J. Monod, Annual Review of Microbiology, 1949, pp. 292 - 347.
As Monod mentions in his paper (The Growth of Bacterial Cultures, Annual Review of Microbiology, Volume 3, 1949, pp 371-394):
........Although the methods of culture will vary according to the problems investigated, certain general requirements must be met in any case. The most important one is that the cultures should be constantly mixed, homogenized, and in equilihrium with the gas phase. This is achieved either by shaking or by bubbling air (or other gas mixtures) or both........
From this it is clear that bacterial culture should be single cell suspended culture.
Best regards
Vit
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