Main problem is that they don't come out with Trypsin.EDTA or Tryple.select or EDTA. I have tried everything. I want to re-plate them for one particular experiment. Can anybody help me in the procedure?
For cells that are strongly adherent and don't come off with standard trypsinization, I wash with Ca/Mg free PBS and use 1mL Accutase for about 10-15 minutes.