does anybody know how to dissolve thiobarbituric acid in 20% TCA for MDA assay? it is not dissolve with me even with warming
Thanks
Use 0.2M HCL and 15% TCA
TCA takes really long to dissolve and re-crystallizes fast, especially when the solution is stored in the refrigerator. Try Mr. Galbraith's suggestion and leave the solution to stir on a magnetic stirrer overnight. That should work.
It is good to bring to pH 4
You need to add phosphoric acid solution with the mixture.
Dissolve in dilute HCl and then bring the pH to 7 with dilute NaOH
A bunch of flower for everybody. I would like to ask about the list of journals which we can publish our paper for free. Thanks in advance. Sincerely Walid
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