Does anybody know how to dissolve thiobarbituric acid in 20% TCA for MDA assay? it is not dissolve with me even with warming?

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Steven galbraith Galbraith

Use 0.2M HCL and 15% TCA

Tea Romih

TCA takes really long to dissolve and re-crystallizes fast, especially when the solution is stored in the refrigerator. Try Mr. Galbraith's suggestion and leave the solution to stir on a magnetic stirrer overnight. That should work.

josé Miguel Alemán Polo

It is good to bring to pH 4

Oday Hasan Ali Al-Jammaas

You need to add phosphoric acid solution with the mixture.

Nikhat Siddiqi

Dissolve in dilute HCl and then bring the pH to 7 with dilute NaOH