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Questions related from Walid Abuelsoud
Dear all, is there a difference between 0.1% and 5% trichloroacetic acid (TCA) when used to extract plant leaves... does increase in TCA concentration affects the composition of the extract? Thank you
03 November 2019 8,684 3 View
Hallo all, my question about the peroxides in plant tissues, are they acid soluble? I know hydrogen peroxide is fairly soluble in aqueous solutions while lipid perixides are soluble in lipids......
03 November 2019 7,648 0 View
I have been doing SOD assay using riboflavin, NBT, Methionine in K-phosphate buffer pH 7.6 and accidentally I forgot to add EDTA in the reaction mixture, as a result the blue colour generation in...
27 November 2017 4,610 1 View
I used amplex red kit for estimation of hydrogen peroxide content in Arabidopsis leaves extracted with k-phosphate buffer pH 6.4. The problem is when I make sample control reaction (i.e. extract +...
28 September 2017 6,819 3 View
I will assay the extract content of hydrogen peroxide using amplex red kit. I would use the same extract for assay of MDA, phenolic and flavonoid content
20 September 2017 3,267 2 View
does anybody know how to dissolve thiobarbituric acid in 20% TCA for MDA assay? it is not dissolve with me even with warming Thanks
16 September 2015 9,158 5 View
Hello everybody, I have a problem assaying PAL activity in Euphorbia plant, when I do the assay I got the abosrbance at 290 nm increased for the first 10 min and then decreased progressively with...
26 May 2015 8,984 6 View
