Hello,

I just started to work with LS180 cells. I used EMEM supplemented with 10% FBS and 1% penicillin-streptomycin to culture them. When I wanted to passage the cells, I realized the majority of the cells have not been attached! So, I decided to re-seed the floating cells in new flask and see whether they are attached or not. After 2 days they were again suspended in the media! To check the viability of floating cells, I used Trypan Blue and counted the percentage of dead cells. As I expected the number of alive cells was less than 50%.

I don't know what is the reason for this happening! These cells are supposed to be adherent, however in my culture, they are not!

Please share with me your opinions if you have experinece.

Many thanks in advance