How can I super impose my 3D structure protein with wild?

Hi I got a 3D protein structures in my research and want to superimpose it with the wild. I am working with SARS-CoV2 and found a deletion that I want to compare with that of Wuhan as a wild. I...

16 December 2020 3,940 5 View

What is the average stove efficiency for household gas stove especially in the Indian subcontinent?

In one paper, I saw it to be about 36%. But as the paper was published in 1997, shouldn't modern household gas stoves be more efficient?

14 December 2020 6,199 6 View

Is there a guide for Aspen Hysys to show unit operation calculations?

For example, let's say if I design a conversion reactor and give inlet temperature,pressure,composition and reaction kinetics then Hysys will show me the outlet conditions. It doesn't,however,show...

09 December 2020 8,375 3 View

How can I convert my amino acid sequence in PDB formate?

I have some sequences of Spike protein of some Covid 19 samples that have been sequenced in my laboratory using NGS. For analyses of those, I need to convert those sequences into PDB format. I am...

01 December 2020 690 3 View

Why I get this type of band after doing PCR?

After doing a PCR with my specific primer I got multiple bands instead of getting a unique band. I am totally astonished after getting this. Can anyone guessed what happened why I get this band...

26 October 2020 3,714 17 View

Which decision rule algorithm is best in machine learning? Is there any algorithm based on rough sets that gives good results?

I am trying to learn some algorithms that extract decision rules from data sets, particularly by using rough sets. I need to know whether they are reliable and efficient (like LEM2) for real datasets?

17 August 2020 7,013 6 View

What is the convenient way to to measure the degree of cooperativity in DNA-DNA or DNA-protein interaction?

Hill equation is used to determine the degree of cooperative behavior in ligand -enzyme interactions. If I would like to utilize this equation in DNA-DNA or protein-DNA interaction, is it...

18 December 2019 5,712 2 View

Why the colony size so small and not cultured in to new LB plate?

New discussion After DNA ligation, I transformed into E. coli and get very small size colony but when I want to make a new LB plate by picking some colony, I did not get any colony culture. I...

04 November 2019 3,275 3 View

Universal sample size calculation through open epi software?

Hi everyone I am doing my research project which is entitled "Knowledge and attitude regarding HPV virus and vaccination against HPV among medical students'. My study population are from 1st year...

26 October 2019 7,128 1 View

Does surface particle size (surface component) has any relation with atmospheric optical depth (atmospheric component)?

I have estimated thermal inertia for a surface followed by its particle size, I have to look for the relation between particle size and atmospheric optical depth, I have gone through the...

11 October 2019 7,986 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View