Need to detect E.coli presence/absence in pork meat samples, no quantification needed. I was thinking in API, but since I have been not able to get the code books I'm not sure if API10S or API20E will be useful. It doesn't works also if its for O157:H7, I don't need so specific analysis, just to know if there is any kind of E.coli.
By the way can somebody upload the API code books please?
By Simple methods, I mean kit-like o really simple bichemical test. I need it for people with no biological formation , so PCR or other molecular technique is totally discarted for them.
Petrifilm, by 3M.
It's really simple and fast. Innoculate and incubate for 24hrs at 35 ºC, the blue colonies with gas bubble are E. coli.
http://solutions.3m.com/wps/portal/3M/en_US/Microbiology/FoodSafety/product-information/product-catalog/?PC_7_RJH9U523003DC023S7P92O3O87000000_nid=C0WJ62882Vbe29BDXSBJ7Fgl
You need to specify whether you are happy to culture or not. Detection without culture will most likely rely on PCR based technology. There are specific primers specified in this paper: http://www.iwaponline.com/jwh/004/0487/0040487.pdf. If you are happy to wait for cultures to grow then either the Colilert by Idexx or 3M Petrifilm based systems as detailed above would be some of the quickest and easiest ways to go. API kits typically require more time as you first need to grow up some isolates then put your suspected E.coli colonies through the API testing. Generally you would isolate on MacConkey agar and test the lactose fermenting oxidase-negative Gram-positive rods Though still requiring the initial isolation steps the API 20E Rapid kit is relatively quick, taking around 4 hours after you have the isolates. See http://delrio.dcccd.edu/jreynolds/microbiology/2421/lab_manual/API%20%20RAPID%2020E.pdf
Petrifilm is a good alternative, in not necessary to preper culture medium, just diluition buffer. The method is sheeper, easy and so fast, the result is in 24-48h from E. coli
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