Sebastian Sarmiento

3 Questions 10 Answers 0 Followers

Questions related from Sebastian Sarmiento

Why does a shadow appear in my Cy3 dyed mouse microarray?

When scanning my microarrays a shadow appeared in the Cy3 Channel (see picture below). I used a mouse microarray from Microarray Inc. and pre-hybridized it. I also washed and dried the array...

03 March 2014 8,268 2 View

Why am I having trouble in trying to measure protein concentration with Bradford after RIPA buffer extraction?

I performed protein extraction from cell pellets using RIPA1X buffer and ROCHE's protease inhibitors cocktail cOmplete. When take it to Bradford protein quantification (Bio-rad) protocol, I get...

13 January 2014 4,190 13 View

Does anybody know a quick, simple method for E.coli detection?

Need to detect E.coli presence/absence in pork meat samples, no quantification needed. I was thinking in API, but since I have been not able to get the code books I'm not sure if API10S or API20E...

16 October 2013 617 5 View