Does anybody know a protocol to deplete lung microbiota? If so, do you know if the protocol modifies intestinal microbiota?
I don't have an answer, but I have the same question. Do you have any clue by now?
Dear Researchers, We are conducting a systematic review to investigate return to work and need the full text of the paper intitled "An open study of diflunisal, conservative and manipulative...
18 November 2018 2,310 2 View
Hi all, I'm trying to clean up some DNA for genomic sequencing (Nanopore and Illumina), but i'm still struggling with the purity of my DNA. My 260/280 ration is ok, that is not an issue, however...
02 March 2018 9,334 3 View
I have extracted DNA from fungal mycelia but when I checked on Nanodrop, most of my samples have a 260/230 ratio < 2. I think the contamination might be from polysaccharides, since some of the...
11 December 2017 7,206 10 View
07 December 2017 657 9 View
Please, I wonder if there are commercially available antibodies and conjugates for the determination of CD4 / CD8 for turtles.
04 March 2015 7,836 2 View
I was wondering if anyone knows or have ever seen a paper correlating the changes from larvae to pupae (in mosquitoes) and the synthesis or degradation of genetic material. I'm asking this because...
10 February 2015 9,501 7 View
Recently scientists changed the classification of important vectors of diseases in the world based of course on new scientific data. For example, Aedes aegypti, responsible for transmitting...
20 February 2014 1,790 4 View
I would like to know if anyone knows a good alternative to an expensive software for qPCR analysis called qBasePLUS by biogazelle. The ones that I found were not good enough.
23 July 2013 208 2 View
I recently started using morphometry in my studies but now that we acquired some new equipment, I'm a little bit lost. Does anybody know how can I convert my pixels to mm? For example, I took a...
21 December 2012 5,705 17 View
Does anyone know a possible way to quantify the nutritional intake of a larvae of mosquito without killing it?
23 July 2012 3,415 10 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...
19 July 2024 3,906 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...
15 July 2024 5,898 0 View