Does anybody know a protocol to deplete lung microbiota? If so, do you know if the protocol modifies intestinal microbiota?
I don't have an answer, but I have the same question. Do you have any clue by now?
Hi all, I'm trying to clean up some DNA for genomic sequencing (Nanopore and Illumina), but i'm still struggling with the purity of my DNA. My 260/280 ration is ok, that is not an issue, however...
02 March 2018 9,231 3 View
I have extracted DNA from fungal mycelia but when I checked on Nanodrop, most of my samples have a 260/230 ratio < 2. I think the contamination might be from polysaccharides, since some of the...
11 December 2017 7,125 10 View
07 December 2017 456 9 View
I would like to know if anyone knows a good alternative to an expensive software for qPCR analysis called qBasePLUS by biogazelle. The ones that I found were not good enough.
23 July 2013 118 2 View
Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
02 March 2021 3,584 1 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View
My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.
24 February 2021 4,150 3 View
I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...
21 February 2021 3,838 1 View