No title

30 August 2020 2,230 5 View

How to get pure recombinant virus with plaque purification ?

We are working on getting pure desired gene transfected virus. We used YFP fluorescences tags to distinguish the virus with or without gene replaced. Using PCR testing, we always have wild type...

01 August 2020 2,087 1 View

How to make Sentaurus silver.par to be the perfect conductor like defined electrode?

I have been trying to create some perfect conductor in my simulation as a connection between 2 spots(different doping semiconductor area). But is seems that the barrier bewteen silver and the...

10 March 2020 2,535 1 View

Do anyone have the experience of testing creatinine (kidney function) by using HPLC?

In my experiment, I want to use serum (mouse) to test the creatinine level via HPLC. 1. During the preparation, I centrifuged the whole blood, and then transfer the supernatant (which is serum)...

17 February 2020 9,679 2 View

What is the best indoor spectrum that people need?

What is the best spectrum for an office, classroom, or other architectural scenes? Spectrum affects people's visual needs, work efficiency, comfort, psychological or physiological rhythm. How to...

20 December 2019 5,715 4 View

How can I remove RNA from IP samples without affecting protein?

09 October 2019 5,713 3 View

What effect does precipitation pH and heat treatment temperature have on the electrical resistance of the material?

I have prepared a series of nanomaterials by co-precipitation under different pH value and heat treatment temperature. I want to find a reasonable explanation about the change of the material...

21 August 2019 9,192 3 View

Sentaurus simulating anti-reflection coating issue?

I am starting to simulate the anti-reflection coating (ARC) for solar cells but I found some problems that I can't answer: 1, For solar cell without ARC, I tried to put the light source below...

03 July 2019 6,682 2 View

How to consider the question of theoretical support for statistical model choosing?

In my research I focus in paired correlated binary data. To accommodate the intraclass correlation, I choose to adopt a certain model without providing much theoretical support, and was criticized...

04 June 2019 7,887 12 View

Why the ZnO I grinded photodegrade slower than bulk?

These days, I do dye degradation experiment. I use mortar and pestle to grind bulk ZnO 10 min, the powders turn yellow. Then use that powder to do dye degradation, why it degrades slower than...

05 August 2018 1,769 4 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View