I am looking for a construct which can be inserted in the intron, which has loxp site and a fluorescent marker to check for the insertion.
We made it.
https://elifesciences.org/content/6/e24635
Journal publication Inter disciplinary area various nationality
05 August 2020 9,144 2 View
In taxonomy research articles, we could see spelling errors in some scientific names of species probably of typographical error or adopting the pioneer work etc., but what causes misrepresenting a...
14 March 2017 765 8 View
24 October 2016 6,404 3 View
I want to modify my protein so that it will be degraded by ubiquitin mediated degradation. What is tag or the sequence i need to fuse with my gene of interest that will lead to K48 (lys48)...
20 February 2016 9,634 2 View
Respected Professors and Friends I want to perform SOFC efficiency test for my powder samples. I my current work I got the conductivity of 0.016 S/Cm at 600 C. So i want to perform the SOFC...
12 January 2016 1,531 4 View
01 January 1970 9,014 3 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.
28 February 2021 5,929 2 View
Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...
24 February 2021 2,006 2 View
I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...
23 February 2021 1,934 1 View
I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...
21 February 2021 6,260 3 View
I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...
20 February 2021 5,820 4 View
Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...
20 February 2021 1,291 3 View