I am looking for a construct which can be inserted in the intron, which has loxp site and a fluorescent marker to check for the insertion.
We made it.
https://elifesciences.org/content/6/e24635
Anyone explain the EDX Spectra and Elemental composition (at. % and Wt. %) of the dopant and composite. Why do we need both percentages? No one explains clearly in the research article....
27 February 2024 1,885 1 View
How to calculate the Atomic and Weight percentage from EDX? Any formula to calculate the at.% and Wt. %?
28 January 2024 3,085 3 View
Kindly explain, how to calculate the elemental composition of weight and atomic percentage from EDX. Does anyone know the formula to calculate the at% and Wt%?
09 January 2024 1,575 3 View
Join us for the NetSim Webinar on Jan 11, 2024, at 15:30 IST, focusing on vehicular ad hoc networks with NetSim & SUMO co-simulation. Learn about advanced techniques and applications in...
07 January 2024 1,602 1 View
Kindly send any one for Standard XPS book in the downloaded format.
13 December 2023 8,697 5 View
What is the conclusion obtained from weight and atomic percentages of elements present in the samples?
13 June 2023 1,709 0 View
13 June 2023 5,796 3 View
What is the physics mechanism behind that??
30 March 2023 6,643 2 View
I need a clear explanation, How to add error values for all the experimental data?
01 February 2023 2,635 3 View
Here's the case , For papers accepted for publication in (JES or JSS, )authors choose whether to publish their articles as open access.(ie via standard subscription mode) All articles accepted...
02 November 2022 1,593 1 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
05 August 2024 853 3 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...
22 July 2024 7,073 2 View
Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...
22 July 2024 8,770 1 View