What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

How can I open a SXV4 file (SuperCROSS) in SPSS, R or STATA?

Hello everyone, I received some data from Statistics South Africa in an SXV4 format (SuperCROSS) and would like to open it in SPSS (or a similar programme if need be). I have tried opening it...

05 January 2021 2,463 4 View

Flow cytometric analysis of NBDG-uptake and intracellular staining possible?

Hello everyone, I am measuring glucose uptake into my cells by using NBDG and flow cytometry. Normally this works perfectly fine, but now I got some problems. When I fixed and permeabilised my...

25 October 2020 3,416 5 View

Why am I getting western bands with white bubbles?

12 October 2020 6,976 3 View

Does anyone know how I can see which nodes I chose for a specific file in NVIVO?

Hello! Does anyone know how I can see which nodes I chose for a specific file in NVIVO? I appreciate your answers, Katharina

02 October 2020 110 3 View

Ion torrent - What happens if the amplicons are too long?

Hello everyone, What happens if I try to sequence with Iron torrent amplicons (eg of a marker gene) that are too long? By that I mean more than 500bp. We did try (not on purpose, we did not...

11 August 2020 368 3 View

How can I analyse the modal ethnicity of a household and compare this to the ethnicity of the household head (SPSS)?

Hello everyone, I am working with a household survey and have both a person and a household file. I am using the household file for the main part of my analysis but want to explore the household...

21 July 2020 7,716 2 View

Who is working with Alzheimer's brain data?

Hi everyone, I am looking for a typical Alzheimer's brain for teaching purposes. I need a .nii file or similar. Of course the file should be showing a very typical brain and must be...

25 November 2019 7,917 3 View

Can we unbias data from umbrella simulations with missing data points?

Imagine a system with 2 energetic basins A and B separated by a high barrier. We cannot sample that barrier for whatever reason (ie. because the lowest energy path is very "long" with lots of...

11 September 2019 9,156 1 View

Can you recommend any peptide-coated microplates?

Hi! Can you recommend any company (or at least a research-team) where I can order a microplate covered with peptides according to my needs? I need it for immunological assays. Thanks for all...

12 February 2019 5,959 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View