Does anybody has experience relaxing chitons before extracting their gonads?

More Margarita Brandt's questions See All

I need to do a qRT PCR but there are not available filter tips?

Due to the COVID situation it is nearly impossible to find filter tips. Is it possible to do it without filter tips by making sure to clean the pipette with RNAse zap before use and working...

17 January 2021 8,839 3 View

Should I move forward on a study if I find there is a weak correlation, but not statistically significant?

I'm conducting a correlational study (Spearman rho) and there are no statistically significant relationships between the variables with a sample size of 37 (4 independent variables/1 dependent...

04 January 2021 5,976 5 View

How can I build a membrane patch with multiple monomers of a peptide?

Hi, I have been looking around for ways to insert various monomers of an AMP in membrane bilayers. To start-off, I have been using Charm-Gui, but it seems that I can only insert one peptide at a...

29 November 2020 8,660 2 View

Time to prepare samples for RNA-seq?

Hello, I am analysing the cost benefit of doing: RNA extraction and library preparations myself Sending RNA samples for sequencing Sending the entire sample for extraction and RNA preparation...

26 November 2020 2,203 2 View

How to analyze Lipid extraction (QDa HPLC) data?

Dear colleagues, I've been working on some lipid extraction analysis with QDa HPLC. I start with some biological samples that are spiked with a different final concentrations of a lipid mix...

14 March 2020 919 4 View

NCI-plot wfn file failure?

Hello, My name is Margarita Bulatova and I am a Ph.D. student from the University of Jyvaskyla, Finland. I hope that there is someone who can help me with my NCI calculations problem. I have...

24 January 2020 7,742 3 View

Doed anyone have a protocol for comet assay in chlamydomonas?

Hi everyone! I am looking for an efficient comey assay protocol in chlamydomonas reinhardtii if you have some suggestions let me know thanks a lot!

15 January 2020 698 3 View

Why do we test the water swelling behavior of a material which is tested for its suitability for bone tissue engineering?

I understand what water swelling behavior means, but how is that related with a materials properties to assist in bone regeneration ?

13 April 2019 4,549 4 View

Is it possible significance results on spearman correlation to be different from the results on a scatterplot graph? and why?

Is it possible that we are missing something?

04 February 2019 8,485 2 View

Commercially available Arc/Arg3.1 antibodies for rat tissue?

Dear all, we are currently looking for a reliable anti-arc/arg3.1 antibodies which will be working in rat organotypic hippocampal slices. Has anyone already tried and know a good ones? We will be...

17 October 2018 9,794 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Ivette Ruiz Boijseauneau

We the best way to relax them has been by cold we placed one trays with sea water in the refrigerator in the most low part between 12 to 24 hours and the organisms die completely stretched

Margarita Brandt

Gracias Ivette!

Anna Smith

I haven't tried this with chitons but you may want to try Tricaine methanesulfonate (MS-222) or clove oil. I had success using both of these on sea stars.

Thank you Anna!

Thank you Tim, I agree with you. We're only trying the cold and our original method to see which one works best. We will only do gonadosomatic index, so I guess either one is OK?