Does anybody happen to know S. epidermidis counts in ready to eat meat products?

More Magdalena Podkowik's questions See All

Has anyone use Emx1Cre adult mice to label pyramidal neurons?

I want to inject a Cre dependent virus to be expressed in pyramidal neurons, and I have Emx1Cre adult mice. I know Emx1 is a developmental gene, but found a paper showing expression of Emx1 also...

28 March 2024 1,666 0 View

Are there any guidelines for using CHAT GPT in your research paper title?

I always take the longest time thinking about titles and writing abstracts, so I think it might be a good use. But I wonder if journals should include this in their guidelines, or else, it's not a...

12 February 2024 7,730 1 View

OMVs labeling for uptake assay?

Good evening, Can you please advice on a protocol for labeling of OMVs for cellular uptake assay. OMVs are derived from gram-negative strain. I was thinking of applying R-18 but other dyes are...

03 October 2023 2,929 3 View

Problem with F-AAS Zinc quality controls standards?

Hello, in our lab we use F-AAS for determination of Mg in urine and Li, Zn in serum. Except Zinc QC 2 everything works fine. Is it possible there is problem with zinc controls? I am waiting for...

04 September 2023 4,169 1 View

Does anyone has a CamKII primary antibody to recommend for immunostaining in slices?

Hi! Im experiencing problems with CamKII immunostaining for free floating mouse PFC and Hp slices. I ve tried different blocking agents ( NDS, NGS, BSA in different percentages), different mouse...

30 July 2023 7,341 0 View

How to CRISPR-KO and CRISPR-HDR of p53 in fibroblasts?

I performed CRISPR-KO of p53 in fibroblasts. I've got cells that lost the activity of WTp53 (several target genes tested on qPCR) but on western blot, I see still quite a high level of a protein...

15 January 2023 8,080 0 View

ImageJ or QuPath?

How can I quantify DAB using ImageJ or QuPath (staining intensity and number of positive cells)?

03 July 2022 6,002 3 View

ReproSil NH2 column for sugars analysis - mobile phase?

I have five different columns from Dr.Maish: 1) ReproSil - XR 120 NH2 2) Reprosil 80 NH2 3) Reprospher 100 CN 4) Reprosil - Pur 120 NH2 5) Reprosil Saphir 100 NH2 4) Reprosil - Pur 120 NH2 Can I...

08 June 2022 2,467 0 View

What polymeric material have the most similar properties as nylon 6.6 , and it is not from the polyamides family?

What polymeric material has the most similar properties as nylon 6.6 in terms of the processing and mechanical properties, and it is not from the polyamides family?

05 August 2021 6,193 8 View

Which method/scale should I use to measure self-disclosure online?

I am currently looking for a scale to measure online self-disclosure. The results will help me write my master's thesis.

13 July 2021 9,306 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View

Simulation of metal drawing by Abaqus with UMAT?

Hello, colleagues. Recently, I have been working on a metal processing simulation with my UMAT in Abaqus. I have outlined the corresponding simulation, but I keep encountering issues that cause...

30 July 2024 7,062 1 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

Does anyone know a source for theta-replicating Staphylococcus plasmids that are compatible?

I am trying to put 3 separate plasmids into Staphylococcus aureus and I am looking for those that are theta replicating and compatible. I have obtained pSK9065 (from pSK1) from Addgene, but the...

23 July 2024 6,968 0 View