The problem is that my Antioxidant solution is soluble in Chloroform, so how can I perform this Assay as standard protocol is to make solution of Ferric chloride, ferry cyanide, trichloroaceteic acid in distilled water only?
I used this article for the antioxidant assays. You can find the attached.
Antioxidant activity must be evaluated under physiological conditions. This means either in water at neutral pH or in nonpolar organic solvents (mimicking lipids) such as alkanes. There is no sense to measure antioxidant activity of compounds dissolved in chloroform. First, make your extract soluble in water or in heptane/octane
Hi, MD,
I agree with Yurii's answer. Antioxidant assays must always be done in physiological pH and conditions, hence mimicking the environment where we theorize it to function.
I suggest that you read more on FRAP in published protocols/articles.
Best,
Arizaldo
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