Hy,

of course you can use primary mouse antibodiesand detect them with secondary anti-mouse in rat tissue.

You can do also the inverse way and use rat primary antibodies in mice.

Good luck

Stefan

Hi Stefan,

Thank you for your answer

Unfortunately, secondary antibodies against murine primary antibodies cross-react with rat antibodies. This results in a nonspecific IHC reaction

Hi Igor Ivanovich Babichenko,

Thanks for your valid point. I should consider that point as well. 

Regards,

Harsha