What is the main advantage of using observer based fault tolerant controller(FTC) for sensor fault tolerance?

I am working on FTC for handling sensor faults in the system. I have read about observer-based FTC schemes which use error threshold-based logic between the states and estimated states. If a...

10 January 2021 5,771 2 View

Can a luenberger observer (even a modified version) be effective for a nonlinear affine system?

I want to know whether a luenberger observer or a modified form of it be effective for estimating the states of a system of the following forms: 1. x_dot = f(x,u,d); y = x 2. x_dot = f(x,u); y =...

20 December 2020 1,392 4 View

Implementing a non-local constraint in COMSOL Multiphysics?

I am trying to simulate a planar, high ohmic DC gas-discharge system. The model consists of a coupled set of equations, drift-diffusion for ions and electrons, and Poisson's equation in the gas...

07 December 2020 8,521 2 View

Is there any software for calculating hit bias (HB), missed precipitation (MP), and false precipitation (FP)?

I have a time series of two rainfall products. For error decomposition analysis, I have to calculate HB, MB and FB. Is there any software for calculating these variables.

27 November 2020 980 2 View

I'm looking for cell culture models for Alzheimer's by targeting amyloid beta peptide, tau protein, bace 1 enzyme ; can anyone give me details please?

I want to target particular protein/peptide/enzyme by cell culture methods. I would also like to know screening methods for cell cultures to analyse and diagnose the drug effect on particular...

24 November 2020 7,255 5 View

What modeling software can be used to analyse fate of pesticides using a fugacity multimedia approach?

Hello, I am trying to understand the fate of pesticides based on the fugacity multimedia approach. Is there software for such modelling? I know SWAT can be used for watershed-level pesticide...

15 October 2020 1,424 3 View

Can someone help me in designing off-MPPT mode in a Standalone DC Microgrid?

I am working on voltage control of a Standalone DC Microgrid consisting of Single PV source, a battery and loads. The PV is interfaced through Buck converter to the DC bus, the battery is...

01 October 2020 7,071 2 View

How to amplify extremely high GC-rich template?

I am cloning a whole cDNA of a particular gene and I have designed forward primer at start of 1st exon. The region downstream of forward primer is extremely high GC rich (around 85-89%). Because...

17 September 2020 6,571 11 View

How to apply load in abaqus ?

I have created a beam and i dont know, what is the maximum load it can take, Is there any way to increase the load continuously without giving a fixed value, so that at some load the beam will...

02 September 2020 6,246 9 View

I have a list of properties of tool steels here. Please let me know which of the below mentioned properties will change after heat treatment?

16 August 2020 730 8 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View