Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Pls inform me if the problem here is in the used red safe. im using the red safe of vivantis co, Malysia. using 5 microliter per 100 ml of agarose?

Why the ladder is always with so closle or attached bands after the 500 bp band whatever i use a type of ladder ( 5types) and also the PCR product is bended. is this has a relation with the used...

05 January 2021 5,211 5 View

Is it important/advisable to write a summary for each theme (and subthemes) in Thematic Analysis?

I am conducting semi-structured interviews with graduate students to explore their academic writing challenges. I am using thematic analysis to analyze the Qual findings. Should I include a...

31 December 2020 3,593 22 View

Using Matlab and the analytical solution for Room acoustic modeshapes, is it possible to plot modes in 3D?

mode shapes similar to attached image.

27 November 2020 4,262 7 View

What tool can I use for creating a Quantative Trait Loci map from SSR data?

I have SSR data for some individual of three hybride How I construct QTL mapping of yield according to these data ?

02 October 2020 502 5 View

Labs for purification, identification & isolation of unknown proteins form native sample of honey and to test versus GS-9L cells in vitro?

I asked for protein purification, identification and isolation of unknown proteins and test versus GS-9L other than that we already got in our patent WO/2014/040605 - form native sample of honey?!

23 September 2020 1,839 3 View

Hi everyone, do you know any GPS logger that we can use to conduct travel time surveys and which is easy to extract the data from ?

We are currently planning to conduct Freight Master Plan in the state of Qatar, one of the requirements is to conduct travel time surveys, therefore we are currently searching for GPS devices that...

22 July 2020 1,889 1 View

How can I quantitatively analyze a sample with carbohydrate content?

Can anyone -who may have hands-on experience in analytical chemistry or photochemistry- help me how can I quantitatively analyze a sample with carbohydrate content?

09 July 2020 3,663 2 View

Any Predictive Markers for Severe COVID-19 in children?

Predictive Markers for Severe COVID-19 Identified

15 June 2020 1,375 5 View

How to critize (Evidence based medicine) this paper?

24 May 2020 5,489 3 View

How to PEGylate Fluorescntl Polystyrene Particle?

I have Fluorescent Polystyrene beads. I need to graft PEG on the surface of it. I am looking for a protocol to do that. Also need to know which experiment to perform to be sure about the grating.

01 December 2020 7,860 1 View

Tail vein versus retro-orbital injection for experimental metastasis assay?

Hi, researcher. It's a bit urgent that I have to inject tumor cells intravenously in 10 days. I am now practicing tail vein injection with a success rate of around 50%. I am still don't have...

20 October 2020 3,572 15 View

BT549- Tail vein injection?

Hi everyone, does anyone have experience with performing tail vein injection with the breast cancer cell line BT549? I am looking for some advice on what the metastasis burden looks like, what...

14 October 2020 215 2 View

How is the quality of archived blood sample for HbA1c test?

HbA1c is an important indicator of long-term glycemic control with the ability to reflect the cumulative glycemic history of the preceding two to three months. Also, HbA1c is measured as the ratio...

16 September 2020 2,423 4 View

How to view accident data in VEINS?

I used the veins default example and when I run the simulation with the accident parameters set, I get the scalar and vector file. How to view the accident data in the output files.

14 August 2020 4,955 1 View

In what cases does blood pass through implants according to the Darcy law?

Hello! I would like to understand in what cases blood or some other biological liquid passes through implant or graft with some porous microstructure according the Darcy law (thanks to the...

26 March 2020 8,670 14 View

Do I need to measure fish blood pressure in quantifying amino acid?

Hi all, I would need to quantify amino acids present in hepatic vein of a fish. Do I also need to record the blood pressure to get the exact AA levels in the fish blood? Thanks.

25 March 2020 3,466 1 View

Any mathematical study for sickle cell disease?

Any mathematical study for sickle cell disease?

21 February 2020 4,857 4 View

Whats your idea about Au mineral and Bi mineral in Quartz veins ?

I saw two minerals in quartz veins (Au mineral and Bi mineral ) in an epithermal deposit. could you please help me how to interpret it.

04 February 2020 1,307 4 View

What is the best way to collect weekly blood samples from mice that are being exercised?

I plan to carry out a study where I run mice on a treadmill for 6 weeks. I would like to have blood samples from before they exercise and at weekly timepoints. Briefly, we want to look at...

01 February 2020 289 1 View