First, do you think it is necessary to dilute blood or bone marrow samples before adding it on lymphoSep to separate the Buffy coat? OR simply add it on lymphoSep and then centrifuged.
Second, what is the preferred speed of centrifuge ?
Rukaiah Al-Ammar
I think you need to diluted
(Lymphocytes are isolated by layering diluted, heparinized blood over a solution of sodium metrizoate that contains a polysaccharide)
Polysaccharide aggregates the erythrocytes so that they can be sedimented by low centrifugation
The less dense lymphocytes remain as a clearly defined band at the plasma Lymphosep interface.
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