Can I use asymmetric PCR amplification on a pool of circular plasmids to increase each plasmid's signal?

Hello, Here is my experiment: I transfect in a plasmid that has been cut once (a linear piece of dsDNA) into human cultured cells. I wait 48hrs to allow the cells to "repair" the plasmid making...

10 February 2021 6,090 3 View

Recommendations for IL-10 Receptor staining?

11 October 2020 9,412 3 View

What is appropriate to use as my affiliation for a manuscript after finishing graduate school?

I would like to publish my graduate (M.S.) research, which is wildlife/conservation-related, and which I conducted at a U.S. university. It's been a few years since I finished graduate school. I...

14 September 2020 6,153 19 View

What marker can I use to analyze dendritic spine maturity on a tissue section?

I would like to quantify the number of dendritic spines in all the maturation stages by using a coronal section of a mouse brain. I will be looking more specifically in the hippocampus.

30 September 2019 7,816 5 View

How do I write a PDB file with custom non-atom particles for a coarse grain model for molecular dynamics simulations (on GROMACS)?

I am trying to write custom particles to a PDB file for coarse grain molecular dynamic simulations in GROMACS. In particular, I am trying to model RNA nucleotides using a handful of beads per...

05 July 2018 7,785 2 View

Uninvited guest in intestinal organoids?

Please excuse the poor photograph. This very large body has appeared in my mouse intestinal organoid culture. To the right (out of focus) is my budding organoid, and in the centre is this very...

14 February 2017 6,791 2 View

What causes huge black dots on (nitrocellulose) membranes?

Dear all, I transferred proteins labeled with a fluorescent dye from (a part of) a 2D SDS-PAGE gel to a nitrocellulose membrane. After scanning the nitrocellulose membrane to check whether the...

27 October 2016 2,816 3 View

Is silver citrate hydrate and/or silver acetylacetonate soluble in ethanol?

For a synthesis I need a silver derivative that is soluble in ethanol. Does anyone have any experience on how well silver citrate hydrate or silver acetylacetonate dissolve in ethanol? It is...

28 July 2016 1,376 3 View

What is the best method to avoid contamination between samples that need to be homogenized prior to DNA extraction?

We have many samples to be homogenized and the material need to be washed between samples to avoid DNA contamination between them. It is hard to effectively bleach all the materials. I did some...

01 May 2016 443 6 View

How can we assess emotional attachment?

Preparing a presentation on the Theory of attachment and would like to have my fellow classmates do an exercice or answer a questionnaire on the subject. Any ideas of the type of questions I could...

29 February 2016 4,022 2 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

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02 March 2021 3,060 3 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

First time secondary research, some points I should keep in mind?

I am conducting secondary research for my dissertation for undergraduation (Psychology) , what should I make sure my proposal consists of? Are there any tips I need to keep in mind?

22 February 2021 6,035 1 View