I made extraction of tryptophan metabolites from frass of cricket Acheta doemsticus. I have used silica plate and TLC to analyse extract composition. As a manual source I used Mullins (1973) TRYPTOPHAN METABOLITE EXCRETION BY THE AMERICAN COCKROACH. On the line of solvent front you can find red dots, Does anyone have idea what it can be? In the article there is no information about it. On the picture you can find two similar plates (on both plates in similar order, from the left: 1-tryptophan, 2 - wild, 3 - yellow, 4-white). Blue spots are respectively, from down: hydroksyquianaldic acid, kynurenic acid, xanthurenic acd. Left plate developed in propanol:2%NH4OH, right plate in butanol:metanol:NH4OH.
Crickets belong to eye-colour mutant strains: Krzyżowski (2016) Differences in stimulated locomotor activity in eye-colored mutant strains of house cricket
Hello,
I think that the red spots are not the metabolites of tryptofan. There can be some compounds from the homogenized frass,differ from tryptofan and its metabolites. For example, intensive red fluorescence can give porphyrin or porphyrin-containing enzyme metabolyzing tryptophan.
Hello,
thank you for help. This is good suggestion, I was thinking about something different than tryptophan, because it was moving with solvent forehead. Maybe this is microbiological metabolite? I asked about it because there is a strange differenece between wild and yellow/white.
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