Hello, I looked through some soil isolation kits but most of them use also mechanical lysis of cells. Do you know about any soil DNA isolation kit giving HMW DNA 50-100 kb long? For example PowerSoil and PowerMax soil kits isolate DNA with maximum length 10-20 kb.
Hi Tomáš,
maybe you can try this kit http://www.roboklon.de/pdf/183_det_en.pdf
Honestly, I never used it but I use the DNA+RNA+Protein purification kit from the same company for extraction of DNA and RNA from water samples filtered on filter membranes and it works very well. I don't know why the columns should not hold back your high kb DNA but you can just ask the company, they are really kind and helpful!
Cheers,
Janina
Hi Tomás,
Why do you say that the Mobio PowerSoil and Power Max kits isolate DNA with maximum length 10-20 kb? Where did you read that? I could not find that information anywhere. I'm surprised because these kits are designed to extract genomic DNA from soil bacteria, which is larger than that. I had good results with soil mobio kits when extracting DNA from soil and concentrated water, but I never checked the length of the extracted DNA.
Cheers,
Helena
Thanks for your reply and sorry for my late answer! To answer your question Helena: I called to the company and ask there to find out the lenght of isolated DNA! They told me that because of harsch methods of lysisof cells, it is not possible to isolate longer fragments...
Hi Tomás, I asked them by e-mail some months ago and they told me the same :) Thanks for your answer!
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